antibody binding sites การใช้

• The presence of large carbohydrate chains extending from gp120 might obscure possible antibody binding sites.
• The labeled antigen competes for primary antibody binding sites with the sample antigen ( unlabeled ).
• This generates two separate monovalent ( containing a single antibody binding site ) Fab fragments and an intact Fc fragment.
• In addition, Protein M has a C-terminal domain with 115 amino acid residues that probably protrudes over the antibody binding site.
• One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule ( containing two antibody binding sites ), hence the designation F ( ab') 2.
• Another biomolecular engineering technique that is used in ELISA development is the bioconjugation of an enzyme to either an antibody or antigen depending on the type of ELISA . There is much to consider in this enzyme bioconjugation such as avoiding interference with the active site of the enzyme as well as the antibody binding site in the case that the antibody is conjugated with enzyme.